Skip to main content
. 2023 Jul 7;11:1212230. doi: 10.3389/fbioe.2023.1212230

FIGURE 2.

FIGURE 2

The 3D-HTLM contains three confluent cell layers and shows high TEER. (A) Graphical representation of the layers of the model. Graphic made with Biorender. (B) DAPI stained model imaged with fluorescence microscopy demonstrates the differences of nuclear morphology of each layer of the model. White boxes indicate the regions of interest, which are shown on the right at higher magnification. Each layer is indicated by the corresponding color in panel (A). (C) TEER of individual and combined layers of 3D-HTLM on day 13. The data set was normalized to measurements obtained with the PET membrane and medium alone. One-way ANOVA with Tukey’s multiple comparisons test. * signifies p < 0.05, ** signifies p < 0.01, *** signifies p <0 .0001; n = 3. (D) H&E staining of paraffin embedded 3D-HTLM with myeloid cells added. The green arrow indicates the PET transwell membrane. The blue, red, and pink arrowheads correspond to the top, middle, and bottom layers indicated in panel (A). Images shown are representative of 30 images taken from three wells.