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. 2023 Jul 7;11:1212230. doi: 10.3389/fbioe.2023.1212230

FIGURE 6.

FIGURE 6

LPS stimulation of the 3D-HTLM leads to increased ICAM-1 expression on CD31+ HPMECs and increased secretion of IL-10, TNF⍺, and IL-6 dependent on myeloid cells. (A) FACs gating strategy to identify CD31+ endothelial cells with or without LPS stimulation. (B,C) Mean fluorescence intensity (MFI) and representative FACs histogram of (B) ICAM and (C) VCAM with LPS stimulation on CD31+ endothelial cells. Paired two-tailed t-test (n = 10) Grey, filled line = no LPS; black, unfilled line = +LPS. (D,E) MFI and representative FACs histogram of (D) ICAM and (E) VCAM with LPS stimulation on CD31− cells. Paired two-tailed t-test (n = 10) Grey, filled line = no LPS; black, unfilled line = +LPS. (F,G) MFI of (F) the activation markers CD86, CD40, and PDL1 and (G) HLA-DR of recovered myeloid subsets with or without stimulation of LPS. Paired two-tailed t-test (n = 6). (H) Multi-plex bead array analysis of the supernatant from 3D-HTLM with or without the addition of myeloid cells and stimulated with LPS. * represents p < 0.05, ** represents p < 0.01. Repeated measure two-way ANOVA with Sidak’s multiple comparisons test.