FIGURE 7.

3D-HTLM models challenged with RSV had increased production of type I IFN and expression of IFN-response genes. (A) Fluorescence microscopy of whole mount models challenged with RFP-RSV. Images shown are representative of 10 images taken from two wells. (B) RT-qPCR analysis of the gene encoding the RSV F protein 24 h after challenge. An aliquot of the whole model digest was used for RNA isolation. (C) Supernatant from RSV-challenged or uninfected models were added to a monolayer of WISH cells for 6 h. IFN-inducible genes, IFIT1, MX1, and PRKR RNA was measured by RT-qPCR. * indicates significance from uninfected control supernatants. Wilcoxon paired two-tailed t-test. (D) Multi-plex bead array analysis of the supernatant from 3D-HTLM with or without the addition of myeloid cells and challenged with RSV. * represents p < 0.05, ** represents p < 0.01, **** represents p < 0.0001. Repeated measure two-way ANOVA with Sidak’s multiple comparisons test.