Fig. 3.

Flow cytometry immunophenotyping of peripheral blood mononuclear cells. Whole blood was collected and first subjected to filtration and subsequently to Ficoll-Paque density gradient separation (A). Flow cytometry immunophenotyping was performed at each step of the procedure, and frequencies of CD14⁺ monocytes, CD3⁺ T lymphocytes and relative CD8⁺ and CD4⁺ subsets and circulating CD34⁺ hematopoietic stem cells were investigated (B). Percentage of each cell subset was compared before and after filtration and after separation to confirm filter efficacy without losing cells of a particular subset during the procedure (C-D). Data are shown as mean ± SD. A p < 0.05 was considered statistically significant.