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. 2023 Jun 9;9(6):e17141. doi: 10.1016/j.heliyon.2023.e17141

Fig. 4.

Fig. 4

Myogenic commitment of bone marrow-derived mesenchymal stem cells (hBM-MSCs) by flow cytometry analysis. BM-MSCs were co-cultured with myoblasts over 21 days, and myogenic commitment was monitored at day 0, 7, 14, and 21 by investigating variations in mesenchymal and myoblastic marker expression. hBM-MSCs and myoblasts alone were employed as control. Protein expression is reported as normalized cell count histograms (a) or as median fluorescence intensity (MFI) (b) for each studied marker and population over the culture period. MFI of each marker was calculated on single cells in each population and time point, and on hBM-MSCs and myoblasts cultured alone. (c) Variations in frequencies of CD90−CD45+, CD90 + CD45+, CD90+CD45− (hBM-MSC-like phenotype), and CD90−CD45− (myocyte-like) cells were investigated throughout the culture, and BM-MSCs and myoblasts cultured alone were used as controls. (d) On myocyte-like population, CD73 and CD105 expression was compared at each time point. Data are shown as mean ± SD. *p < 0.05; **p < 0.01; ****p < 0.0001.