Fig. 5.

Gene expression profiling for myogenic markers by quantitative RT-PCR ofhBM-MSCs-hSkMs-PBMCs in static culture.hBM-MSCs were co-seeded with hSkMs in ratio 2:1 and PBMCs were culture in the upper chamber of transwell inserts. mRNA levels of myogenic markers: Pax3, MyoD1, Myf5, Myf6, Desmin and MYH2 were assayed by qRT-PCR at day 7, 14 and 21 of culture. The relative quantification of each mRNA gene expression normalized to endogenous GAPDH (internal control) was calculated using the 2^−ΔΔCt method and presented as fold change over hBM-MSCs at day 0, selected as a control.