Fig. 7.

Flow cytometry immunophenotyping of peripheral blood mononuclear cells (PBMCs) in transwell. First, CD4⁺, CD3⁺CD4⁻CD56⁻ (assumed as CD8⁺ T lymphocytes), CD3⁻CD56⁺ Natural Killer (NK), and CD3⁺CD56⁺ NKT cells were identified (a), and perturbations were monitored throughout the culture (b). On CD4⁺ and CD8⁺ T cells, T central memory (TCM), T naïve (TN), effector memory cells re-expressing CD45RA (TEMRA), and effector memory T (TEM) cell subsets were studied (c) and monitored along the culture (d-e).