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. 2023 Jun 10;9(6):e17194. doi: 10.1016/j.heliyon.2023.e17194

Fig. 6.

Fig. 6

Regulation of MMP1 expression by PRKRA via NF-κB pathway. (A) The phosphorylation of NF-κB was detected by Western blot after PRKRA knockdown and overexpression. (B) The changes in MMP1 mRNA levels after treatment with TNFα and PDTC were detected by RT-qPCR. (C–D) The MMP1 mRNA and protein levels could be recused by TNFα and PDTC after PRKRA knockdown or overexpression. (E–F) Cistrome and JASPAR database showed potential binding of P65 to the MMP1 promoter region. (G) ChIP was performed with an anti-P65 antibody in AsPC-1 and MiaPaCa-2 cells. (H–I) Dual luciferase assays were applied to confirm the binding site of P65 to the MMP1 promoter region in AsPC-1 and MiaPaCa-2 cells. (*p value < 0.05; **p value < 0.01; ***p value < 0.001; ****p value < 0.0001.)