Figure 2. GLUT2 Gene Silencing Effects on Male versus Female Primary Astrocyte Culture Mean Phospho/Total MAPK/ERK1/2 Protein Ratios.

Figures 2A and 2B depict mean ratios of phosphorylated versus total 44 or 42 kDa ERK1/2 protein expression, respectively, in SCR or GLUT2 siRNA-pretreated male (gray bars) versus female (white bars) astrocytes incubated with media containing or lacking glucose. Mean ratio data were analyzed by three-way ANOVA and Student-Newman-Keuls post-hoc test, using GraphPad Prism (Volume 8) software; results are presented in Supplementary Table 1. Open circles depict individual independent data points. Statistical differences between discrete pairs of treatment groups are denoted as follows: *p < 0.05; **p < 0.01; ***p < 0.001.