FIG. 1.

(A) Sequences of the N-terminal tags of the purified fusion proteins used in this study. Tags A1 to A4 are encoded by pET19b-HMK and pJS124. Slightly different versions of the tag were generated as a result of cloning from different sources. In tag B, the HMK sequence has been eliminated in the cloning protocol (see Materials and Methods). The HMK recognition sequence is a phosphorylation site for the catalytic subunit of bovine HMK. (B) Sequence of ParB. The arrows indicate the N terminus of each proteolytic fragment identified in this work. Bands A to E were generated by trypsin digestion. Band F was produced by chymotrypsin digestion.