FIG. 3.

Chymotrypsin and trypsin digestion of His-ParB. (Left) Five micrograms of protein was incubated with increasing amounts of protease for 2 h at room temperature. Protein-to-protease ratios (wt/wt) were 100:1 (lanes a and i), 500:1 (lanes b and h), 1,000:1 (lanes c and g), and 5,000:1 (lanes d and f). Lane e, no protease. Arrows indicate the fragments whose N termini were sequenced. (Right) Tryptic digest (protein/protease ratio of 1,000:1) performed at 30°C for 7.5 h, illustrating an additional band (E) that was also sequenced. The positions of size markers are indicated beside each gel. Tryp, trypsin.