FIG. 5.

Examples of filter tests to determine β-galactosidase activity in the yeast two-hybrid system. The light patches were white and the grey patches were blue on the filters. Each panel (A and B) is a separate filter, but the patches within each panel are from the same filter. The particular interactions tested are indicated at the right of each filter. Various ParB and ParB fragment interactions (A and B) and ParA-ParB interactions (B) are shown. GAL4-ACT is the GAL4 activation domain alone encoded by pACTII and represents one of the negative controls for these assays.