TABLE 1.
Vectors and P1 plasmids
| Vector | Description | Marker(s)a | Reference |
|---|---|---|---|
| pAS1 | GAL4 DNA binding domain vector | TRP1, Apr | 16 |
| pACTII | GAL4 activation domain vector | LEU2, Apr | 16 |
| pET19b-HMK | Protein expression vector; N-terminal 10-His tag; HMK phosphorylation site | Apr | 8 |
| pJS124 | pET19b-HMK derivative; stop codons inserted in all 3 frames at BamHI site | Apr | This study |
| pJS9 | parB in pBluescript SK+ | Apr | This study |
| pJS10 | parB in pBluescript SK+; opposite orientation of pJS9 | Apr | This study |
| pJS49 | pJS10 with BglII site immediately upstream of parB | Apr | This study |
a
Apr indicates ampicillin resistance in E. coli. TRP1 and LEU2 are nutritional markers in S. cerevisiae.