TABLE 2.
Plasmids for yeast two-hybrid analysis and for protein expression
| Plasmid | Vector | ParB fragmenta |
|---|---|---|
| For yeast two-hybrid analysis | ||
| pJS50 | pAS1 | FL-ParB |
| pJS51 | pACTII | FL-ParB |
| pMR2 | pAS1 | 30-333 ParB |
| pMR4 | pACTII | 30-333 ParB |
| pJS37 | pACTII | 47-333 ParB |
| pJS38 | pACTII | 67-333 ParB |
| pJS39 | pACTII | 87-333 ParB |
| pJS40 | pACTII | 93-333 ParB |
| pJS41 | pACTII | 187-333 ParB |
| pJS44 | pACTII | 275-333 ParB |
| pJS45 | pAS1 | 275-333 ParB |
| pJS141 | pACTII | 1-312 ParB |
| pMBP1 | pACTII | 1-293 ParB |
| pJS136 | pACTII | 1-277 ParB |
| pJS139 | pACTII | 1-234 ParB |
| pJS140 | pACTII | 1-189 ParB |
| pJS138 | pACTII | 1-177 ParB |
| pJS137 | pACTII | 1-128 ParB |
| pJS182 | pACTII | 1-61 ParB |
| pJS181 | pAS1 | 1-61 ParB |
| pBEF217 | pACTII | FL-ParA |
| For protein expression | ||
| pJS12 | pET19b-HMK | FL-ParB (tag A1) |
| pJS117 | pET19b-HMK | 47-333 ParB (tag B) |
| pJS118 | pET19b-HMK | 67-333 ParB (tag B) |
| pJS119 | pET19b-HMK | 87-333 ParB (tag B) |
| pJS120 | pET19b-HMK | 93-333 ParB (tag B) |
| pMD11 | pET19b-HMK | 152-333 ParB (tag A2) |
| pMD12 | pET19b-HMK | 175-333 ParB (tag A2) |
| pJS121 | pJS124 | 187-333 ParB (tag A3) |
| pMD13 | pET19b-HMK | 267-333 ParB (tag A2) |
| pJS123 | pJS124 | 275-333 ParB (tag A3) |
| pJS172 | pJS124 | 1-312 ParB (tag A4) |
| pJS151 | pJS124 | 1-293 ParB (tag A4) |
| pJS128 | pJS124 | 1-277 ParB (tag A4) |
| pJS189 | pJS124 | 1-274 ParB (tag A4) |
| pJS146 | pJS124 | 1-245 ParB (tag A4) |
| pJS164 | pJS124 | 1-234 ParB (tag A4) |
| pJS143 | pJS124 | 1-189 ParB (tag A4) |
| pJS144 | pJS124 | 1-188 ParB (tag A4) |
| pJS145 | pJS124 | 1-182 ParB (tag A4) |
| pJS129 | pJS124 | 1-177 ParB (tag A4) |
| pJS166 | pJS124 | 1-128 ParB (tag A4) |
| pJS149 | pJS124 | 1-114 ParB (tag A4) |
| pJS148 | pJS124 | 1-61 ParB (tag A4) |
| pJS198 | pJS124 | 47-177 ParB (tag A3) |
a
Sequences of the N-terminal tags are shown in Fig. 1A.