Fig 2. hbxA is required for normal conidiation and for production of ST and other secondary metabolites in A. nidulans.

(A) Cultures of wild type, deletion, complementation and overexpression hbxA strains, top-agar inoculated on glucose minimum medium (GMM) and incubated for 7 days in the dark at 37°C. (B) TLC analysis of extracts from top-agar inoculated cultures incubated for 3 days in the dark. Black arrows indicate ST standard. The experiment was carried out with three replicates. (C) Densitometry of TLC analysis of ST levels in (B). The densitometry was performed using the http://biochemlabsolutions.com/GelQuantNET.html website. Error bars represent the standard error. Columns of different letters represent values that are statistically different p value of <0.05.