Skip to main content
. 2023 Jul 21;18(7):e0286271. doi: 10.1371/journal.pone.0286271

Fig 9. hbxA regulates the production of austinol and dehydroaustinol in A. nidulans.

Fig 9

Wild type (WT), deletion (ΔhbxA), complementation (hbxA-com), and overexpression (OEhbxA) strains were top-agar inoculated on solid glucose minimum medium (GMM) at 37°C for 72 h. Samples were collected, extracted with chloroform and analyzed by LC-HRMS in positive mode. Quantification of (A) austinol (m/z 459.20059) and (B) dehydroaustinol (m/z 457.18524) compounds by full MS spectra resolution of 60,000 with a range of mass-to-charge ratio (m/z) set to 50 to 800. (C & D) Heatmap of TPM values of austinol cluster (DEGs) expression in A. nidulans ΔhbxA and OEhbxA with respect to wild type strain on a log scale found in Inglis et al. [50]. The TPM value of each gene was calculated by averaging all the TPM values of all replicates.