FIGURE 2.

Cycle-dependent performance of antibodies. Human lymph node histologic slides were stained with CD66b at a dilution of 1:600 for 30 minutes at room temperature at cycle 1 (A) and cycle 9 (B). In addition, human lymph node samples were stained with CD141 at a dilution of 1:100 overnight at 4°C at cycle 2 (C) and at cycle 9 (D). After testing, it was determined that these antibodies cannot be used at later cycles within these specific staining conditions. Subcapsular region is shown in both A and B. Consecutive sections are shown in C and D. Top row scale bar: 100 μm (5X magnification).