Figure 1.
Identification of scRNAseq cell clusters in children with MtD and controls. (A) Peripheral blood mono nuclear cells were collected from children with MtD (N = 4) and matched controls (N = 4). Single cells were sorted, followed by isolation of RNA and sequencing. (B) Log10 cell numbers for analyses from children with MtD and controls. (C) Differential gene expression analyses identified cell specific markers. A dot plot for cellular marks and their alignment with various cell populations was constructed. (D) Table of RNA markers of various immune cell subsets. (E) UMAP clustering of immune cell populations as identified using selected marker genes. (F) Feature plots showing the distribution of cellular markers for the identification of immune cell populations.