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. 2023 Jul 7;14:1142634. doi: 10.3389/fimmu.2023.1142634

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Copyright © 2023 Gordon-Lipkin, Banerjee, Franco, Tarasenko, Kruk, Thompson, Gildea, Zhang, Wolfsberg, NISC Comparative Sequencing Program, Flegel and McGuire

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lymphoblastoid cell lines express MTRNR2L8 and mir4485 under conditions of bioenergetic stress. (A) Spare respiratory capacity by extracellular flux analysis (N = 5/group). (B) ATP production by extracellular flux analysis (N = 5/group). (C) MTRNR2L8 expression in lymphoblastoid cell lines cultured in galactose to promote OXPHOS. (D) mir4485 expression under galactose conditions. (E) mir4485 knockdown using antisense oligonucleotides. CTRL = transfection with scrambled antisense. (F) Staining for live cells by flow cytometry. (G) Staining for dead cells by flow cytometry. (H) Staining for apoptosis by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.