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. 2023 Jul 21;9(29):eadh0102. doi: 10.1126/sciadv.adh0102

Fig. 3. BCAS increases the number of GFAP-positive astrocytes in WT but not TRPA1-KO mice.

Fig. 3.

(A to D) Representative images of immunostaining with anti-Iba1 antibody in the corpus callosum (A) and (C) and summarized data for the number of Iba1-positive cells (B) and (D) on days 14 (A) and (B) and 28 (C) and (D). (E to H) Representative images of immunostaining with anti-GFAP antibody in the corpus callosum (E) and (G) and summarized data for the number of GFAP-positive cells (F) and (H) on days 14 (E) and (F) and 28 (G) and (H). (I) A schematic of quantitative RT-PCR of the corpus callosum. (J) Gfap mRNA expression in the corpus callosum on day 14. Values are means ± SEM. Scale bars, 100 μm. (B) n = 13 to 15; (D) n = 14 to 16; (F) n = 8 to 10; (H) n = 6 to 11; (J) n = 9 to 12 (WT), n = 12 to 13 (TRPA1-KO). *P < 0.05 and ***P < 0.001 for two-way ANOVA with Bonferroni’s post hoc test (D), (F), and (H). *P < 0.05 for two-tailed unpaired Student’s t test (J).