. 2023 Jul 21;9(29):eadf1402. doi: 10.1126/sciadv.adf1402

Table 1. Comparison of K_ds.

The dissociation constant (K_d) between CXCR4^QTY and its binding partners obtained from device models agree with those from biological assays. GFET, graphene-based field-effect transistor.

Ligand	Biological assay (nM)*		GFET device test (nM)
Ligand	Native	QTY	Langmuir fitting model
CXCL12 buffer	∼5	17.3 ± 4.2	20.1 ± 9.1
CXCL12 serum†	∼5	31.4 ± 12.2	24.8 ± 13.0
gp_41–120 buffer	∼200‡	7.0 ± 1.9	5.8 ± 3.1
gp_41–120 serum†	∼200‡	12.7 ± 2.6	19.1 ± 6.1

*K_d values from biological assays were obtained from our previously published paper (16).

†Because of the technical implementation, biological assays were conducted in 50% human serum, while the device tests were conducted in 100% human serum.

‡K_d between native CXCR4 and HIV coat glycoprotein was measured by a cell-based assay.

Table 1. Comparison of Kds.

Table 1. Comparison of K_ds.