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Lytic effect of the expression of the S. aureus murE gene in E. coli cells. Cells of BL21(DE3)/pLysS/pMuSA2 were grown exponentially at 37°C in 2YT-ampicillin medium. At the time indicated by the arrow (OD = 0.4), IPTG was added at a final concentration of 1 mM. Growth of cells induced (○) or not induced (●) with IPTG was monitored at 600 nm.
