TABLE 1.
Pool levels of peptidoglycan precursors, peptidoglycan content, and specific activities of MurE enzymes in E. coli cells harboring the pMuSa2 plasmida
| Peptidoglycan precursor, peptidoglycan, or enzyme | Pool level or sp actb in:
|
|
|---|---|---|
| Noninduced cells | Induced cells | |
| Peptidoglycan precursors | ||
| UDP-GlcNAc | 160 | 370 |
| UDP-MurNAc | 90 | 90 |
| UDP-MurNAc-pentapeptide(A2pm) | 1,230 | 400 |
| UDP-MurNAc-pentapeptide(lysine) | 150 | 1,710 |
| Peptidoglycanc | 8,370 | 6,120 |
| Enzymes | ||
| A2pm-adding enzyme | 2.1 | 2.1 |
| Lysine-adding enzyme | 0.07 | 23.4 |
a
Cells of BL21(DE3)/pLysS(pMuSa2) were induced or noninduced with 1 mM IPTG for 1 h, as described in Materials and Methods. In each case, all of the parameters were tested from samples of the same culture.
b
The pool level (for peptidoglycan precursors and peptidoglycan) is given in nanomoles per gram (dry weight) of bacteria. The specific activity (enzymes) is given in units per milligram of protein.