Figure 1.

A) Approximately 31% of HA repeat units were successfully modified with NB, as determined by proton NMR analysis performed in deuterium oxide. ¹H NMR shifts of pendant norbornenes at δ6.33 and δ6.02 (vinyl protons, endo), and δ6.26 and δ6.23 ppm (vinyl protons, exo) were compared to the HA methyl group δ2.05 ppm to determine functionalization. Reprinted from Acta Biomaterialia, Volume 150, Alexa R. Anderson, Ethan Nicklow, Tatiana Segura, Particle fraction is a bioactive cue in granular scaffolds, Copyright 2022, with permission from Elsevier.¹² B) Schematic of the flow-focusing microfluidic device used to generate HA-NB μgels. C) Maximum intensity projections from confocal microscopy were used to visualize fluorescently labelled μgels (scale bar = 500 μm). D) Frequency distributions of microgel diameter from independent runs on the microfluidic setup demonstrate control over microgel size ~50 μm or ~100 μm depending on the device used. E) Microgel diameter is reported as the mean and standard deviation for each independent run. Reprinted from Advanced Materials, Volume 34, Issue 33, Wilson, et al., Stoichiometric post-modification of hydrogel microparticles dictates neural stem cell fate in microporous annealed particle scaffolds, Copyright 2022, with permission from Wiley.⁹