Figure 2.

A) Maximum intensity projection of μgels in aqueous solution (scale bar = 100 μm). B) Purified μgels can be incubated 1:1 by volume in the lyophilization medium of choice and lyophilized. C) Maximum intensity projection of dried lyo-μgels (scale bar = 100 μm). D) Microgels are resuspended after lyophilization. 70% EtOH is recommended for retaining the μgels original properties throughout the lyophilization process; however, other mediums such as isopropyl alcohol (IPA), water, and acetonitrile (MeCN) can be used interchangeably to facilitate cryogel formation (scale bar = 100 or 50 μm as noted). E) Measurement of HA-NB microgel diameter before (gray) and after lyophilization (green) in 70% EtOH shown as frequency distributions for three microgel populations. Reprinted from Acta Biomaterialia, Volume 150, Alexa R. Anderson, Ethan Nicklow, Tatiana Segura, Particle fraction is a bioactive cue in granular scaffolds, Copyright 2022, with permission from Elsevier.¹²