Figure 3.

A) Schematic of HA-NB μgels being interlinked with a tetrazine linker to form MAP scaffolds. B) Reaction scheme for HA-Tet synthesis. C) The HA-Tet reaction was setup and allowed to react overnight followed by D) precipitation of HA-Tet in ethanol. E) Once purified and dried, the HA-Tet was rehydrated and lyophilized to yield F) a dried, light pink product. G) Proton NMR analysis shows successful modification of 11% of HA repeat units. Reprinted from Acta Biomaterialia, Volume 150, Alexa R. Anderson, Ethan Nicklow, Tatiana Segura, Particle fraction is a bioactive cue in granular scaffolds, Copyright 2022, with permission from Elsevier.¹²