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. Author manuscript; available in PMC: 2024 Jul 21.
Published in final edited form as: Circ Res. 2023 Jul 6;133(3):271–287. doi: 10.1161/CIRCRESAHA.121.320324

Figure 3. p53 deletion in fibroblasts leads to an exaggerated fibrotic response in left ventricle pressure overload.

Figure 3.

A) Heart sections were obtained from mice of indicated genotype at baseline, 14 days, or 28 days post-TAC, and stained with PicroSirius Red to visualize collagen fibrils. B) Quantification of PicroSirius Red staining from images in (A). For each genotype, n= 4 (day 0); n=5 (day 14); n=12 (day 28). C) Heart sections were obtained from mice of indicated genotype at 28 days post-TAC. Representative images of sections stained with Biotin-conjugated collagen hybridizing peptide (CHP, purple) to visualize denatured collagen as an indicator of active tissue remodeling, and wheat germ agglutinin (WGA, blue) to label cell membranes. D) Quantification of CHP staining from images in (C). n=9 (WT), 17 (HET), 8 (KO). Data is represented as Mean +/− SEM. Datapoints indicate results from individual mice. Data in is analyzed by Kruskal-Wallis test followed by Dunn’s test to calculate pairwise comparisons. Scale bar = 150μm (A) or 50μm (C).