Figure 1. Use of shRNA to reduce virus production by hexon knockdown. A, Kinetics of virus production in 293T cells modified to express shRNA. The data are reported as means±SD of β-gal positive cells from 3 independent experiments. *P<0.05 (two-way ANOVA with Bonferroni post-test). B, Relative expression levels of adenoviral hexon targeted by shRNA in modified 293T cells upon infection with recombinant adenoviral vector Ad-LacZ. The data are reported as means±SD from 5 independent biological experiments. *P<0.05 vs 293T; #P<0.05 24 h vs 36/60 h (two-way ANOVA with Bonferroni post-test).
