Figure 3. Reduced adenovirus production in cell clones with hexon knockdown. Cell lines were infected with recombinant adenoviral vector Ad-LacZ, MOI of 1, and virus-containing lysate was collected 60 h later. An equal volume of each lysate was used to transduce 1×105 HT1080 cells and 48 h later these were (A) fixed and stained with X-gal or (B) incubated in the presence of the colorimetric substrate, O-nitrophenyl-beta-D-galactopyranoside (ONPG). The data are reported as means±SD of 3 independent experiments. *P<0.05 vs 293T (one-way ANOVA with Bonferroni post-test). a.u.: arbitrary units.
