Figure 5. Reduced adenovirus production upon hexon knockdown. A, Transmission electron microscopy (scale bar 0.5 μm). For each cell line, 5x105 cells were infected with recombinant adenoviral vector Ad-LacZ, MOI of 1, and 36 h later fixed for electron microscopy. In the electron photomicrographs, C indicates cytoplasm, N indicates nucleus, and arrowheads indicate adenovirus particles. B, Quantification of virus particles seen in 5 fields from each of 8 virus-positive nuclei were counted in two independent biological experiments and data are reported as means±SD. *P<0.05 vs 293T (Student's t-test). C, Quantification of infectious virus particles after full scale production and purification. IVP: infectious virus particles. *P<0.05 vs 293T (Student's t-test).
