Table 3. Cytokinesis-block micronucleus test performed with HepG2 cells exposed to different concentrations of Arianor Ebony dye (treatments).
| Treatments | MN | NBUDs | NPBs |
|---|---|---|---|
| Negative control | 31.6±2.51 | 6.0±2.64 | 1.33±0.57 |
| Positive control | 87.3±4.04* | 12.0±2.00 | 10.67±3.21* |
| 50 μg/mL | 83.0±9.53* | 8.0±3.00 | 7.67±3.05 |
| 25 μg/mL | 80.0±4.58* | 17.0±6.10* | 7.67±4.04 |
| 12.5 μg/mL | 81.0±3.21* | 11.33±5.03 | 4.33±1.52 |
Data are reported as mean and standard deviation. Experiments were performed in triplicate. *P<0.05 compared to the negative control (NC) (ANOVA followed by Tukey's test). MN: micronucleus; NBUDs: nuclear buds; NPBs: nucleoplasmic bridges; Positive control: 1% Triton X-100.