Figure 3. The positivity of indirect immunofluorescence-antineutrophil cytoplasmic antibodies (IIF-ANCA) tests using standard and improved techniques in each disease. The overall positivity rate improved significantly from the standard to the improved technique (A) [10/61(16%) to 21/61(34%)]. Although the positivity rate of IIF-ANCA tests remained stable in granulomatosis with polyangiitis (GPA) patients (B) [8/18(44%) to 9/18(50%)], it improved in microscopic polyangiitis (MPA) patients (C) [0/6 (0%) to 1/6 (17%)], in eosinophilic granulomatosis with polyangiitis (EGPA) patients (D) [0/5 (0%) to 1/5 (20%)], in type 1 autoimmune hepatitis (AIH) patients (E) [0/12 (0%) to 3/12 (25%)], and in ulcerative colitis (UC) patients (F) [1/19 (5%) to 7/19 (37%)]. Statistical analyses were performed with McNemar's test.