Table 5. Relationship between indirect immunofluorescence (IIF) results and ELISA results ANCA detection before and after improvements in IIF techniques.
| Standard technique | Improved technique | |||
|---|---|---|---|---|
| IIF-Patterns | PR3-ANCA+ | MPO-ANCA+ | PR3-ANCA+ | MPO-ANCA+ |
| Negative | 7/52 | 2/52 | 5/40 | 1/40 |
| C-ANCA | 2/6 | 0 | 4/14 | 1/14 |
| P-ANCA | 0/3 | 0/3 | 0/4 | 0/4 |
| A-ANCA | 0/0 | 0/0 | 0/3 | 0/3 |
ANCA: antineutrophil cytoplasmic antibodies; MPO: myeloperoxidase; PR3: proteinase 3; C-ANCA: cytoplasmic ANCA; P-ANCA: perinuclear ANCA; A-ANCA: atypical ANCA.