Figure 4. Differentiation of adipose-derived mesenchymal stromal cells into chondrocytes, adipocytes, and osteocytes. A, Control - culture of ADSC without induction and staining. B, Chondrogenic differentiation - confirmed by blue staining with Alcian Blue of GAG proteins. C, Adipogenic differentiation - presence of red lipid droplets after Oil Red O staining. D, Osteogenic differentiation - confirmed by specific staining of calcium deposits with Alizarin Red S (scale bar 50 μm).