Figure 1. Design of sequence-specific shRNAs targeting LMP1. TW03-LMP1 and HNE1-LMP1 cells were treated with sequence-specific shRNAs targeting LMP1. A, Diagrammatic representation of the mRNA encoding full-length LMP1 sequence (CDS). The shRNA targeting the 117-135 base pair (bp) of LMP1 CDS is named LMP1-shRNA1, while the shRNA targeting 1135-1153 bp of LMP1 CDS is named LMP1-shRNA2. B, mRNA expression of LMP1 in TW03-LMP1 and HNE1-LMP1 cells under the administration of lentivirus containing shcontrol, LMP1-shRNA1, and LMP1-shRNA2 sequences. C, LMP1 protein expression in TW03-LMP1 and HNE1-LMP1 cells after the administration of lentivirus containing shcontrol, LMP1-shRNA1, and LMP1-shRNA2 sequences. GAPDH was also assayed as the loading control.
