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. 2023 Jul 7;11:1184799. doi: 10.3389/fcell.2023.1184799

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Copyright © 2023 Chi, Li, Cao, Guo, Han, Zhou, Zhang, Li, Luo, Li, Rong, Zhang, Li and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes of nrg1 and ret knockdown zebrafish. (A) qRT-PCR analysis of relative expression levels of nrg1 and ret in wildtype, nrg1 ^−/−, ret ^−/−, and nrg1 ^−/− ret ^−/− zebrafish embryos at 72 hpf. (B) The survival and deformity rate of wildtype, nrg1 ^−/−, ret ^−/−, and nrg1 ^−/− ret ^−/− zebrafish at 72 hpf. (C) Whole-mount in situ hybridization in wildtype, nrg1 ^−/−, ret ^−/−, and nrg1 ^−/− ret ^−/− embryos at 72 hpf for nrg1, ret, and sox10. White arrows indicated the end of the positive expression of each gene. (D) Immunofluorescence staining and quantitative analysis of a neuronal marker HuC/D in wildtype, nrg1 ^−/−, ret ^−/−, and nrg1 ^−/− ret ^−/− embryos at 72 hpf.