FIGURE 2.

Endogenous HURP spatial dynamics after photobleaching half of the mitotic spindle. (A) Schematic representation of spatial dynamics analysis. SiR-tubulin signal was used as a mask for metaphase spindle compartmentalization in six regions. The fluorescence decay curves of the unbleached spindle half and the fluorescence recovery curves of the bleached spindle half were generated. Bold-lines indicate the mean value and dashed-lines represent the ± S.D. (B) Fluorescence recovery half-lives were quantified by one-phase exponential fitting. (control, n = 16 cells; Nocodazole, n = 13 cells). Bars represent the mean value ± S.D. (C) Fluorescence decay half-lives were quantified by one-phase decay fitting. (control, n = 17 cells; Nocodazole, n = 13 cells). Bars represent the mean value ±S.D.