TABLE 1.
Information of gRNA oligos used to generate the eGFP-HURP HeLa Kyoto cell line.
| Name | Locus | Sequence | Plasmid |
|---|---|---|---|
| gRNA oligo sense | gRNA_HURP_46s | caccgAAGACATCCTGTCAAGGAAA | gRNA_HURP_46s and gRNA_HURP_46a oligos |
| gRNA oligo antisense | gRNA_HURP_46a | aaacTTTCCTTGACAGGATGTCTTc | annealed and cloned into the px335 plasmid |
| gRNA oligo sense | gRNA_HURP_94s | caccgCACATTTTGCCAGTCGACAC | gRNA_HURP_94s and gRNA_HURP_94a oligos |
| gRNA oligo antisense | gRNA_HURP_94a | aaacGTGTCGACTGGCAAAATGTGc | annealed and cloned into the px335 plasmid |