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. 2023 Jul 6;14:1204512. doi: 10.3389/fmicb.2023.1204512

Table 4.

Bioaccumulation of copper in maize leaves under different treatments and its effect on manganese and zinc concentration.

S. No Treatment Cu concentration (mg kg−1 Dry weight)* Mn concentration (mg kg−1 Dry weight)* Zn concentration (mg kg−1 Dry weight)*
1 ST (EGC) @250 mg L−1 21.210 ± 0.593k 6.407 ± 0.546de 12.390 ± 0.162ghi
2 ST (EGC) @500 mg L−1 25.690 ± 0.421j 6.687 ± 0.748de 11.970 ± 0.359hi
3 ST (EGC) @1,000 mg L−1 65.110 ± 0.404i 3.187 ± 0.141g 9.850 ± 0.203jkl
4 ST (CuNPs) @1,000 mg L−1 64.517 ± 0.619i 5.953 ± 0.290e 9.625 ± 0.169kl
5 ST (EO) @100 mg L−1 9.413 ± 0.465l 7.280 ± 0.398de 15.225 ± 0.398d
6 FS (EGC) @250 mg L−1 76.687 ± 2.067h 9.973 ± 0.458bc 14.595 ± 0.571de
7 FS (EGC) @500 mg L−1 140.777 ± 0.566f 6.440 ± 0.081de 12.635 ± 0.613fgh
8 FS (EGC) @1,000 mg L−1 329.827 ± 4.099c 5.543 ± 0.242ef 10.885 ± 0.429ijk
9 FS (CuNPs) @1,000 mg L−1 306.430 ± 1.961d 8.217 ± 0.217cd 13.580 ± 0.199efg
10 FS (EO) @1,000 mg L−1 9.060 ± 0.323l 12.493 ± 0.604a 14.093 ± 0.088def
11 ST+FS (EGC) @250 mg L−1 97.380 ± 1.961g 6.965 ± 0.273de 11.270 ± 0.076hij
12 ST+FS (EGC) @500 mg L−1 180.640 ± 0.374e 3.815 ± 0.264fg 8.575 ± 0.187l
13 ST+FS (EGC) @1,000 mg L−1 375.795 ± 0.177b 3.780 ± 0.210fg 8.645 ± 0.193l
14 ST+FS (CuNPs) @1,000 mg L−1 399.950 ± 1.957a 3.500 ± 0.150g 14.595 ± 0.107de
15 ST+FS (EO) @1,000 mg L−1 8.717 ± 0.061l 9.663 ± 0.291bc 14.595 ± 0.107de
16 Absolute control (Water Spray) 10.570 ± 0.205l 13.300 ± 0.153a 23.975 ± 0.158a
17 Manc @2,000 mg L−1 10.957 ± 0.205l 10.535 ± 0.247b 17.500 ± 0.203c
18 NC (Pathogen only) 11.353 ± 0.267l 12.600 ± 0.351a 19.425 ± 0.122b

*Data are the mean of two different seasons with three replications. Data (mean ± standard errors) followed by different letters in each column indicate a significant difference (Tukey HSD, p ≤ 0.05). ST, seed treatment; FS, foliar spray; EGC, EO-grafted CuNPs; EO, essential oil (clove oil); Manc, mancozeb 75% WP; NC, negative control.