TABLE 1.

Bacterial strains and plasmids used

Strain or plasmid	Description	Reference or source
Strains
E. faecalis
OG1X	str gel	29
JH2-2	rif fus	32
FA2-2	rif fus	14
DS16	Clinical isolate; carries pAD1 and pAD2	14
39-5	Clinical isolate; carries pPD1 and 5 other plasmids	51
FA373	FA2-2 carrying pAM373; Tn918(tet) on chromosome	12
FA3328	OG1X eep mutant (OG1X containing integrated pAM3328)	This study
FA3328R	Revertant of FA3328 that had lost erm	This study
FA3329	OG1X eep mutant (OG1X containing integrated pAM3329)	This study
FA3329R	Revertant of FA3329 that had lost erm	This study
E. coli DH5α	F− φ80 lacZΔM15 endA1 recA1 hsdR17(rK− mK+) supE44 thi-1 ΔgyrA96 (ΔlacZYA-argF)U169	Promega Research Labs
Plasmids
pAD1	60 kb; encodes hemolysin/bacteriocin (cytolysin); responds to cAD1; originally found in DS16	14
pAM2011	pAD1::Tn917lac with insert 4 bp downstream from t2	41
pAM2011E	Miniplasmid derivative representing large EcoRI fragment of pAM2011	46
pPD1	Encodes bacteriocin; responds to cPD1 pheromone	51
pOB1	Encodes hemolysin/bacteriocin; responds to cOB1 pheromone	39
pCF10	Carries Tn925 (tet); responds to cCF10 pheromone	19
pAM373	Encodes response to cAM373 pheromone	12
pAM401	E. coli-E. faecalis shuttle; cat tet	50
pBluescript SK+ II	E. coli plasmid vector; amp	Stratagene
pVA891	E. coli plasmid vector; erm cat	34
pAM3325	pAM401 derivative with 3,045-bp cloned from E. faecalis OG1X	This study
pAM3326	pBluescript derivative with 3,045-bp fragment subcloned from pAM3325	This study
pAM3327	pAM401 derivative with PCR product representing ORF1 (eep)	This study
pAM3328	pVA891 derivative with PCR fragment representing internal segment of eep	This study
pAM3329	pVA891 derivative with PCR fragment representing internal segment of eep (fragment in opposite orientation compared to pAM3328)	This study