Figure 3. Representative z-section from a STEM tomogram of a 750-nm-thick tangential section of a WT mouse retina.

The retina was contrasted with tannic acid/uranyl acetate. Full tomogram is shown in Figure 3—video 1. Because individual rods in the mouse retina are not perfectly aligned, adjacent cells are sectioned at different compartments, including the inner segment (1), outer segment base (2) and mature outer segment (3). Shown on the right is an example of a longitudinally sectioned rod, in which these three locations are depicted by dashed lines. Surfaces of fully enclosed discs are pseudo-colored in magenta to highlight the structure of incisures. Yellow arrowhead points to an incisure in a fully enclosed disc. Tomogram pixel size is 3 nm; scale bars: 2.5 µm (left) or 0.5 µm (right).

Figure 3—video 1. Tomogram associated with Figure 3.

Download video file ^{(11.8MB, mp4)}

Field of view: 12.0 µm x 12.0 µm.