Video 3. Upper video: 2D time-lapse movie of Abp1-mCherry (red in merge) and GFP-Tlg2p (green in merge) in wild-type cell.

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Arrows indicate movement of an Abp1-mCherry-labeled endocytic vesicle toward the GFP-Tlg2p-labeled sub-compartment. Lower video: 2D time-lapse movie of Abp1-mCherry (red in merge) and GFP-Tlg2p (green in merge) in the arp3-D11A mutant. Arrows indicate movement of the GFP-Tlg2p-labeled sub-compartment toward an Abp1-mCherry-labeled endocytic vesicle.