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. 2023 Jul 5;12:e83385. doi: 10.7554/eLife.83385

Figure 5. KD of ATP synthase subunit B and beta reduced both fractional shortening and F-actin staining.

(A–C)Hand4.2-Gal4; tdtK driven KD of ATP synthase subunits at 1 week of age measuring (A) diastolic diameter, (B) systolic diameter, and (C) fractional shortening. Data is plotted as  ± SEM and significance indicated relative to ControlGD2. ****p ≤ 0.0001, **p ≤ 0.01. (D) Quantification of ATP levels from hearts of 1-week-old flies (10–12 hearts per sample). ATP measurements were plotted relative to protein content. (E) One-week-old Hand4.2-Gal4; tdtK driven KD of ATP synthase subunits with altered F-actin (Chchd3/6 KD is depicted to contrast the structural phenotypes). Statistical differences were calculated by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons.

Figure 5.

Figure 5—figure supplement 1. Cardiac KD of ATP synthase components disrupted or diminished F-actin staining.

Figure 5—figure supplement 1.

Chchd3/6 KD in all muscle cells reduced the viability and climbing ability of the fly. (A) KD of mitochondrial ATP synthase subunits using Hand4.2-Gal4; tdtK produced strong F-actin phenotypes. 20 µm scale. (B & C) Chchd3/6 was knocked down using a Mito::GFP; Mef2-Gal4 line; note that Mito::GFP; Mef2-Gal4>Chchd3/6RNAiA is pupal lethal. (B) Viability was significantly reduced in male and female Chchd3/6RNAiC1 flies over a 4-week time course. Mantel-Cox test. (C) Negative Geotaxis assay (average number of flies above a 10 cm mark after being tapped down in a long vial) measured in 1-week-old male and female flies. Unpaired two-tailed t-test, ****p≤0.0001; error bars represent SEM.