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. 2023 Jul 21;12:e83861. doi: 10.7554/eLife.83861

Figure 2. Angular velocity during LRO fluid flow recovery.

Angular velocity polar plots for 6 ss, 7 ss, and 8 ss for the three different groups (A) 'Sham’ control embryos colour coded in green, (B) ‘No LR Defects’ group of embryos colour coded in blue and (C) 'LR Defects’ group of embryos colour coded in red. LR defects refer to misplacement of heart or liver encountered at the larval stage after fluid extraction was performed at 5 ss; number of tracks refers to the number of particle trajectories identified for the quantifications and respective angular velocity plots. Colour code on polar plots refers to the median angular velocity for pooled embryos. (D–E) Violin plots showing quantifications of angular velocities found for the tracks analysed (D) anterior-posterior and (E) left and right. Dots contained in the violin plots correspond to median values per embryo. A statistical linear mixed effects regression was applied (see results in Table 1).

Figure 2—source data 1. Angular velocity quantifications at 6, 7, and 8 somite stages after fluid extraction at 5 somite stage.

Figure 2.

Figure 2—figure supplement 1. Tangential velocity component of particle movement in the outer LRO luminal space.

Figure 2—figure supplement 1.

Tangential velocity polar plots for 6 ss, 7 ss, and 8 ss for the three different groups (‘Sham’ control, ‘Defects’ and ‘No Defects’ group) – number of tracks refers to the number of particle trajectories identified for the quantifications and respective tangential velocity plots. Colour code on polar plots refers to the median angular velocity for all pooled embryos. LRO: left-right organizer; ss: somite stage.
Figure 2—figure supplement 2. Radial velocity components of particle movement in the outer KV luminal space during fluid recovery.

Figure 2—figure supplement 2.

Radial velocity polar plots from the outermost area of the KV (radius > 0.5 for a maximum of 1) are at 6ss, 7ss. and 8ss are represented for the three different groups (‘Sham’ control, ‘Defects’ and ‘No Defects’ group) – number of tracks refers to the number of particle trajectories identified for the quantifications and respective velocity plots. Colors on circular plots refer to the median velocity component for all pooled embryos. Positive radial components indicate predominant flow movement towards the KV surface. ss: somite stage.
Figure 2—video 1. Example of a manipulated embryo that developed normal zebrafish situs (left heart and left liver).
Download video file (5MB, mp4)
Sequence of processed bright-field images of a 6 ss KV is shown, oriented with anterior to the top and left to the left of the page, played at 25 fps (related to Figure 2B).
Figure 2—video 2. Example of a manipulated embryo that developed abnormal situs.
Download video file (2.8MB, mp4)
Sequence of processed bright-field images of a 6 ss KV is shown, oriented with anterior to the top and left to the left of the page, played at 25 fps (related to Figure 2C).