Figure 5. LRO fluid content does not affect left-right development.

(A) dand5 mRNA expression pattern in wild-type control embryos, embryos with LRO fluid diluted with DB and embryos with LRO fluid diluted with MC. Embryos were manipulated at 5 ss and fixed at 8 ss for dand5 whole mount embryo in situ hybridization. (B) Heart position (left, central or right sided) was scored at 30 hpf in wild-type control embryos, embryos with LRO fluid diluted with DB and embryos with LRO fluid diluted with MC. ss: somite stage; DB Danieau buffer; MC methylcellulose; hpf: hours post fertilization.