Table 2. Linear mixed-effects regression: Results for Danieau’s buffer (DB) and methylcellulose (MC) dilution experiments.

Fixed effects coefficient estimates are given. Number of observations: 16622; n=30 embryos; Fixed effects coefficients: 12; Random effects coefficients 90; Covariance parameters: 7. Results relate to Figure 4C–D. Regression formula: AAV ~1 + Intervention*LR +Intervention*PA +Intervention*Time + (1+Intervention | EmbryoID). *** indicates p<0.001; ** indicates p<0.01; * indicates p<0.05.

Name	Estimate	Lower CI	Upper CI	p-value
(Regression line intercept)	0.25148	0.20891	0.29405	6.8705E-31 ***
DB injection	0.070724	–0.030824	0.17227	0.17223
MC injection	–0.12344	–0.19256	–0.05431	0.00046626 ***
Left-right axis	0.0042626	–0.008177	0.016702	0.50182
Posterior-anterior axis	0.16135	0.14901	0.17369	4.4137E-142 ***
Somite stage	0.021413	0.013687	0.029139	5.6349E-08 ***
Interaction: DB injection and left-right axis	–0.012356	–0.027636	0.002925	0.11301
Interaction: MC injection and left-right axis	0.026366	0.008478	0.044254	0.0038681 ***
Interaction: DB injection and posterior-anterior axis	–0.02139	–0.036997	–0.00578	0.0072282 ***
Interaction: MC injection and posterior-anterior axis	–0.073208	–0.092273	–0.05414	5.4857E-14 ***
Interaction: DB injection and time	–0.012635	–0.022248	–0.00302	0.0099937 ***
Interaction: MC injection and time	–0.0004407	–0.012174	0.011293	0.94131