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. 2023 May 30;213(2):221–234. doi: 10.1093/cei/uxad060

Figure 7.

Figure 7.

Hyperthermia downregulates CD55 expression via HSPA5/p65 signaling to activate complement cascade. (A) HONE1 cells were cultured at 37°C or 43°C HT for 1 day or 3 days. The surface expression of CD55 was analyzed by flow cytometry in HONE1 cells. (B) SUNE1 cells transfected with si-NC or si-HSPA5 were cultured at 37°C or 43°C HT for 1 day or 3 days. The surface expression of CD55 was analyzed by flow cytometry in HONE1 cells. (C, D) GSEA was performed using RNA-seq data of HONE1 cells treated with or without HT to identify the gene set enrichment. (E) HONE1 cells were cultured at 37°C or 43°C HT for 1 day, with or without HSPA5 knockdown. The protein expression of HSPA5, p65, phosphorylation of p65 (p-p65), CD55, and GAPDH was analyzed by western blot. (F) HONE1 cells were cultured at 37°C or 43°C HT for 1 day, with or without p65 inhibitor BAY-11-7821 treatment. The protein expression of HSPA5, p65, phosphorylation of p65 (p-p65), CD55, and GAPDH was analyzed by western blot.