Figure 2.

(A) Synthetic route for conjugating multiple peptide chains together at the branch points of 5A1 or 5A2 via CuAAC click chemistry, as exemplified for Con_5A2-(4A)₂. (B) Mass spectrometry data for Con_5A2-(4A)₂. Expected mass: [M+H]⁺/1=15171, [M+10H]¹⁰⁺/10=1518, [M+11H]¹¹⁺/11=1380, [M+12H]¹²⁺/12=1265, [M+13H]¹³⁺/13=1168, [M+14H]¹⁴⁺/14=1085, [M+15H]¹⁵⁺/15=1012, [M+16H]¹⁶⁺/16=949, [M+17H]¹⁷⁺/17=893, [M+18H]¹⁸⁺/18=844, [M+19H]¹⁹⁺/19=799. (C) CD spectra of the four different conjugated peptides, Con_5A1–4A (upper left), Con_5A1–4B (upper right), Con_5A2-(4A)₂ (lower left) and Con_5A2-(4B)₂ (lower right). The subscripts indicate the number of peptide units. Schematic representation of each conjugated peptide is inserted to the corresponding CD spectrum. The weak signals at 208 nm and 222nm indicate the expected absence of coiled coil assemblies.