Table 5.

Analytical Eco Scale assessment of the greenness of the presented and the reported methods using

The developed Method		The reported HPLC method [46]
Reagents	PPs*	Reagents	PPs*
NH4Fe(SO4)2 (< 10 g)	1	DNFB (< 10 mL)	8
		NaHCO3 (< 10 g)	1
Sodium salicylate (< 10 g)	1	KH2PO4, CH3COONa (< 10 g)	0
		HCl (< 10 mL)	4
HNO3 (< 10 mL)	4	Methanol (10–100 mL)	12
		THF (< 10 mL)	6
Ʃ	6	Ʃ	31
Instruments	PPs	Instruments	PPs
Spectrofluorometer	0	HPLC	0
Occupational hazards	0	Occupational hazards	3
Waste (˂10 mL, No treatment)	6	Waste (˂10 mL, No treatment)	6
Ʃ	6	Ʃ	9
Total	12	Total	40
SCORE: 88		SCORE: 60

*Calculations of penalty points (PPs) were performed per sample, DNFB: dinitrofluorobenzene, THF: tetrahydrofuran

Total PPs = Ʃ_{PPs, reagents} + Ʃ _{PPs, instruments}. SCORE = 100 – total PPs