Fig. 4.

Cytotoxic activity of knockout CD19-CAR-NK92 cells versus human primary B cells. A Specific lysis (%) of primary B cells by wildtype (white), reference (green), FasL-ko (blue), Prf1-ko (red) and FasL- & Prf1-ko CD19-CAR-NK92 cells (yellow) at E:T ratios of 1:1. Cells were co-incubated for 4 h. Primary B cells were isolated from blood of two healthy donors. Again, two clones of each knockout type were tested, and results were combined into one data group. Data values are expressed as means of multiple determination ± SEM. P values were calculated by one-way ANOVA followed by Tukey’s test. B Proportion (%) of perforin/granzyme pathway, FasL/FasR pathway and others of the total cytotoxic efficacy of CD19-CAR-NK92 cells against primary B cells at E:T ratios of 1:1. Calculation of proportions as described in legend of Fig. 3